Diets one and two were isocaloric, defined as within 10% of the total energy intake of each other, taking into account differences in energy intake requirements between groups. Amino acids contain nitrogen, which if not incorporated into the body's structure, is converted into urea to be safely excreted. This finding has generated substantial discussion, however, it has often lacked clarity and context, with the term ‘high-protein’ being used unqualified. Athletes require higher protein intake to support muscle repair and recovery, but the balance of macronutrients is crucial. Research suggests that an optimal balance of macronutrients—protein, healthy fats, and carbohydrates—promotes testosterone synthesis. Protein provides essential amino acids that support muscle growth and repair, indirectly influencing testosterone levels. Very high protein diets that significantly reduce fat and carbohydrate intake can lead to hormonal imbalances. At a bare minimum, all authors must be certified nutrition coaches by either the National Academy of Sports Medicine, International Sport Sciences Association, or Precision Nutrition. She has a passion for training, teaching, and writing. Lauren Graham is a Precision Nutrition Level 1 certified nutrition coach. The effect of statins on testosterone in men and women, a systematic review and meta-analysis of randomized controlled trials. This study looked specifically at supplementation with whey protein powder. This would lead to higher cortisol and lower testosterone. A macronutrient ratio that is a majority protein would put a large strain on the body in terms of energy production. This is absurdly high and far beyond what we recommend in our macronutrient breakdowns since protein is the least efficient way for the body to get energy. Dietary intake of up to 35% of calories from protein was considered "moderate protein." By their definition, an extremely low-carb diet would have to mean either a high-fat or high-protein diet (or both). Similar efforts have been made in regard to low-carbohydrate diets (Noakes and Windt, 2017), which also suffer from a plethora of definitions. The terms high-, moderate-, and low-protein diet have no unified definition, and thus without this, remain inherently subjective. ‘High-protein’ has no standard definition relating to the amount of dietary protein (Westerterp-Plantenga, 2007), leaving it open to interpretation when used in isolation. If sleep stays strong and training feels better, coffee is helping your bigger goal. The risk case is that it chips away at sleep and recovery, which can pull testosterone down over time.